Gamme Protein Forest
Protein Forest's digital ProteomeChip™ 
Data quality is greatly affected by upstream mass spec sample processing and by data analysis. The digital ProteomeChip™ system improves both sample prep and data analysis by providing new technology and associated software that insures the highest level of discovery power from your mass spec investment.
Protein Forest® is dedicated to the development, manufacture and marketing of leading-edge products to boost the researcher's ability to correlate protein expression, post-translational modifications and isoforms with disease.
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- Exploit isoelectric focusing of proteins or peptides
- Generate reproducible results
- Analyze high molecular weight proteins
- Achieve 105 depth of coverage
- Streamline your data analysis
- Enjoy Rich data sets for proteomics discovery
Easy to Use
The dPC™ protocol is simple and straightforward. There are no complicated or tedious steps. Researchers of all disciplines will have success quickly with the system.
- Simple straightforward protocol
- Fewer steps, less chances for error
- All necessary reagents are provided in the dPC™ analysis kit
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Compatibility
The dPC™ platform is compatible with a wide array of sample types, workflows and instrumentation. The seamless integration of the technology insures that the benefits of high resolution fractionation and reproducibility provide for an accelerated pace of discovery.
1. Compatible with both complex and simple sample types
2. Get richer, more impactful data from your current efforts
3. Confirm results with isoelectric Western Blotting iWB™
Sample Types
- Human Cell Lysates
- Bacterial Cell Lysates
- Tissue Lysates
- Yeast Cell Lysates
- Plasma
- Urine
- Cerebrospinal Fluid
- Complex Mixtures
- Proteins
- Membrane Proteins
- Peptides
Technology Overview
Unleashing the Power of Discovery
Rich, high quality mass spectrometry data is greatly affected by sample processing upstream to the instrument as well as by the analysis methods used afterwards. The dPC™ platform seamlessly integrates with your current mass spec workflow, giving superior reproducibility, depth of coverage and a powerful tool to streamline data analysis. This ultimately allows the researcher to quickly identify key features (e.g. post-translational modifications, biomarkers, high molecular weight proteins, 550 kD) from robust and reproducible data within minutes after database searching is complete.
The dPC™ Fractionation Technology
The dPC™ separates complex biological samples into fractions suitable for mass spectroscopy, chromatography and blotting using the principle of parallel isoelectric focusing. Proteins or peptides will be charged in an environment that is either above or below their isoelectric point allowing them to migrate in an electric field. Charged proteins/peptides will migrate back and forth through the dPC™ chip between the acidic and basic sides of the chip until they encounter a gel plug that is at or near their isoelectric point where they will become neutral. The uncharged protein or peptide will no longer migrate and will become enriched and separated from other proteins with slightly different isoelectric points.
| Proteins/peptides at pH higher than pI (basic) will be negatively charged. Proteins/peptides at pH below their pI (acidic) will be positively charged. Proteins/peptides at pH equal to their pI will be uncharged |
| Charged proteins/peptides migrate through the dPC™ chip between the acidic and basic sides until they encounter a gel plug whose pH is at or near their pI. The uncharged protein/peptide will no longer migrate and become "trapped" in these plugs. |
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dPC™ Fractionator
MSRAT software |
