Luciferase ELISA Kit

Luciferase is a generic name for enzymes, commonly found in nature, that produce light.  The most commonly used Luciferase is produced by, Photinus pyralis, the firefly.  Luciferase catalyzes a chemiluminescent oxidation–reduction reaction and although many kits are available to detect the enzymatic activity of this protein using light emission, these methods have several drawbacks.  These assays have rapid decay kinetics; require the enzymatic activity of the Luciferase to be retained and they do not enable absolute quantification.  In response to these issues, BIOO has developed a highly sensitive ELISA assay which allows the researcher to completely measure the amount of firefly Luciferase in cell or tissue lysates.

This ELISA Kit provides all of the components needed for rapid, and precise, quantification of firefly, Photinus pyralis, Luciferase in cultured mammalian cells or tissues. 

MaxSuppressor™ Luciferase ELISA Kit as compared to a
Luciferase Enzymatic Activity Assay. 

A luciferase expression plasmid driven by the CMV promoter was transfected into HEK293 cells using Polyethyleneimine (PEI).  Forty eight hours following transfection, the cells were harvested and tested using either the MaxSuppressor™ Luciferase ELISA Kit or the Luciferase Enzymatic Activity Assay.  The data shows that a similar response was observed using both approaches.  However, unlike the Luciferase Enzymatic Activity Assay the MaxSuppressor™ Luciferase ELISA Kit does not depend on active enzyme and can be more accurately quantified.

 

Reference	Description	Qté	Prix unitaire Euros HT
3420-02	MaxSuppressor™ Luciferase ELISA kit	1 kit	493.00 EUR