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Electrophoresis reagents and kits

 >>Sample prep
 >> Staining
 >> Gels
 >> Acrylamide
 >> Ampholytes
 >> Running and Casting Buffer
 >> IEF first dimension buffer
 >> Sample prep buffer
 >> Other electrophoresis reagents and buffers

 

 

  

 

 

GELS

On demand, we can produce pre-cast gels for all kind of electrophoresis systems.

  • low fluorescence glass plate
  • gel thickness 1 mm
  • Plates dimensions:
    • ETTAN DALT: height 21,0 cm x width 27,7 cm and height 21,7 cm x width 27,7 cm;
    • Investigator: height 22,9 cm x width 27,9 cm;
    • Protean II: height 20cm x width 20 cm and height 22, 3cm x width20 cm
  • gel stability : 2 months at +4°C
  • Homogenous or gradient gels
  • differents types of Acrylamide : Mix of standard acrylamide, Duracryl ( high tensile strenght acrylamide), Acrylamide with PDA ( to improve constrat of silver stained gels).

Specific production on request.

 

Pre-cast gels

(22 cm x 22 cm) , for Genomic Solutions Investigator™ and Pharmacia IsoDalt™.

 

Part number
Description
Documentation
Homogenous Gels

0080-0112

Tricine Chemistry /10% Duracryl (10T, 2.2C) (5/pack)
0080-0222 Tricine Chemistry /12% Duracryl (12T, 2.2C) (5/pack)
0070-3923 Tricine Chemistry /10% Duracryl 4% stacker 16 well (5/pack)
0080-0212 Laemmli Chemistry/15% Duracryl (15T, 2.2C) (5/pack)
Gradient Gels

0070-3716

Laemmli Chemistry/8-18% Duracryl (2.2C) (5/pack)

Double Slab Gels

fits Protean IIT (18 cm x 20 cm x 1 mm). Plates dimensions: top 20 x 20 cm, thickness 3 mm; bottom 22.4 x 20 cm, thickness 3 mm.

0070-3696

Tricine Chemistry /10% Duracryl (10T, 2.2C) (5/pack)

0080-0223 Tricine Chemistry /12% Duracryl (12T, 2.2C) (5/pack)
Kits

0070-3925

 

Homogenous kit includes :

  • 2 Pre-Cast Gels [Tricine Chemistry/10% Duracryl (10T, 2.2C)]

  • Tris/ Tricine SDS running buffer (makes 3 L of 1X running buffer Tricine Chemistry) (0080-0115):
 

0070-3715

Pre-Cast Gradient Gel Kit includes:

  • 2 Pre-Cast Gels (18 cm x 20 cm x 1 mm) Laemmli Chemistry, 8-18% Duracryl (2.2C)

  • Tris/ Glycine/ SDS running buffer (0800-0079) (Makes 1.2 L of a 10x solution)
 

 

Pre-Cast Gels - Acrylamide

Référence
Description
Documentation
Single Gels

0080-0213

Laemmli Chemistry/10% Acrylamide (10T, 2.6C) (5 per pack)
0080-0214 Laemmli Chemistry/12% Acrylamide (12T, 2.6C) (5 per pack)
Double Gels

0080-0215

Laemmli Chemistry/Double 12% Acrylamide (12T, 2.6C) (5 per pack)

 

 

Tube Gels

Genomic Solutions Pre-Cast IEF Carrier Ampholyte Tube Gels offer a convenient alternative to casting your own tube gels for 2-D electrophoresis. Our combination of proprietary gel chemistry with a patented threaded tube design yields optimum resolution, reproducibility and ease of use.

Analytical Tube Gels
The pre-cast IEF tube gels come ready to use in 1 mm x 26 cm threaded glass tubes. No rehydration is necessary. The threaded analytical tubes are specifically designed to allow easy gel handling after extrusion from the tubes. The patented thread does not bind protein and has no effect on the protein 2-D pattern. The analytical tubes will accept sample protein loads of up to 100 ? g and sample volumes of up to 50 ? l. Pre-cast tube gels are compatible with any electrophoresis system that can accommodate the tubing sizes.

 

Référence
Description
Documentation

0080-0118

Analytical Pre-Cast pH 3-10 Carrier Ampholyte IEF Tube Gels, 1.0 mm ID x 18 cm (6 per pack)  

0070-3572

Analytical Pre-Cast pH 4-8 Carrier Ampholyte IEF Tube Gels, 1.0 mm ID x 18 cm (6 per pack)

 

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