Emetteurs nanoES
Work online or offline using High quality emitters
In nano-electrospray MS, optimal performance generally requires flow rates from 10 nL/min to 300 nL/min. The very narrow orifices of Proxeon emitters (1 - 30 µm) ensure that such low flow rates can be achieved.
Proxeon emitters and ion sources together facilitate using the lowest possible flow rate, thereby conserving sample while still obtaining a strong signal. Their design takes advantage of the fact that the signal intensity observed in ES-MS is concentration dependent and not a function of total sample consumption.
Working online or offline?
For busy laboratories analyzing large numbers of samples, online nano-electrospray analysis, using stainless steel emitters, is the first choice, facilitating automation and ensuring a higher throughput.
Offline analysis has several advantages when needing to extract the maximum information from very limited amounts of sample. Data can be averaged extensively to improve the S/N-ratio and conditions optimized for MS/MS experiments. Other technical advantages include:
- Work at low flow rates of 10-40 nL/min.
- Utilize nearly 100 % of sample
- Work effectively with sample volumes down to 300 nL.
- Minimize instrument contamination.
- Avoid cross contamination by using disposable emitters
- Spray from purely aqueous as well as purely organic solvents
- Work with stable sprays over a wide pH range and in high buffer concentrations
Performance of nanoES products working offline
Spectrum showing fragmentation of a doubly charged tryptic peptide at m/z 582.33 from 25 fmol loaded on a polyacrylamide gel. After in-gel digestion, the peptides were purified on a micro-column in a purification capillary (ES385) and eluted into a Nano ES emitter (ES380).
Online Emitters
Offline Emitters |
