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Consumables
 

 

Electrophoresis reagents and kits

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 >> Staining
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 >> Acrylamide
 >> Ampholytes
 >> Running and Casting Buffer
 >> IEF first dimension buffer
 >> Sample prep buffer
 >> Other electrophoresis reagents and buffers

 

 

  

 

Consumables > Electrophoresis reagents and kits >

Miscellaneous reagents for Electrophoresis

 

Part Number
Description
Documentation
MSDS
0080-0082 Bromophenol Blue, 5 g
 
 
Catalysts
0080-0173 Ammonium persulfate, 100 g
 
80-0173_APS
0080-0090 Ammonium persulfate, 25 g
 
80-0090_APS
1092356BS Ammonium persulfate molecular biology grade 100 g
 
 
1092391BS Ammonium persulfate molecular biology grade 1kg
 
 
0080-0088 TEMED, 5 ml
 
0080-0174 TEMED, 25 ml
 
80-0174_TEMED
20102399BS TEMED molecular biology grade 10ml
 
 
20102339BS TEMED molecular biology grade 50ml
 
 
Detergents
0080-0080 SDS, 100 g
 
0080-0175 SDS, 1000 g
 
19822359BS SDS, molecular biology grade 500g
 
 
19822391BS SDS, molecular biology grade 1kg
 
 
19812301BS SDS 20% molecular biology grade 1L
 
 
19812335BS SDS 20% molecular biology grade 1L
 
 
0080-0176 Octyl B-D Glucopyranoside, 10 g
 
80-0176_OBG
0080-0177 Octyl B-D Glucopyranoside, 50 g
 
80-0177_OBG
0080-0073 CHAPS Detergent, 10 g
 
0080-0072 Triton X-100, 100 ml
 
0080-0178 Triton X-100, 1 L
 
20182332BS Triton X-100 molecular biology grade 250 ml
 
 

0080-0076

Micro-Clean Detergent, 500 ml : The cleanliness of glass plates is very important. To ensure good results, soak the glass electrophoresis plates overnight in the Micro-Clean detergent cleaning solution.
 

Standards

0080-0321 E. coli Lysate, 500 µl
 
 
0080-0092 2-D Analysis Rat Fibroblast Cell Standard (Qualitative), 150 µl
 

0070-3512

re-cast SDS-PAGE Molecular Weight Marker, 1mm ID x 1cm

These pre-cast markers were developed to allow tracking of molecular weights on 2-D gels. The pre-cast mini-tube gels contain approximately 1.2 micrograms of total protein for a final concentration of 0.1 micrograms per band. Following electrophoresis, the bands can be visualized with the Investigator Silver Stain Kits. These markers are not present in high enough concentration to be detected with Coomassie Blue Stain. The protein molecular weight standards are provided as a 1mm diameter tube gels, approximately 1cm long. To apply the markers to a 2-D gel, simply grasp a mini-tube gel with forceps and place at one edge of the 2-D gel, next to the 1-D gel.

Composition of Markers:

  • Myosin-MW 205,000
  • Beta-Galactosidase-MW 116,000
  • Phosphorylase B-MW 97,000
  • Transferrin-MW 80,000
  • BSA-MW 66,000
  • Glutamate dehydrogenase-MW 55,000
  • Ovalbumin-MW 45,000
  • Carbonic Anhydrase-MW 30,000
  • Trypsin inhibitor-MW 21,000
  • Lysozyme-MW 14,000
  • Aprotinin-MW 6,500
 
0080-0016

Broad Range Molecular Weight Standard
The broad-range standards include 11 proteins from 6,500 to 205,000 Daltons. The mixtures are designed to give sharp, well-separated bands used to determine the molecular weight of proteins run in neighboring lanes of the same gel. The broad-range standards are appropriate for all homogenous polyacrylamide gels or gradient gels. Following electrophoresis, the bands can be visualized with Colloidal Coomassie Blue Stain, SYPRO Ruby Protein stain or a the Silver Stain Kit.

 

Bulk Chemicals

1232389BS Ammonium sulfate molecular biology grade 5kg
 
 
1232391BS Ammonium sulfate molecular biology grade 1kg
 
 

5412399BS

Ethidium Bromide salt, molecular biology grade 500g
 
 

5452341BS

Ethidium Bromide 200µg/ml, molecular biology grade 25ml
 
 
0080-0089 Dithiothreitol (DTT), 5 g
 
80-0089_DTT
0080-0181 Dithiothreitol (DTT), 25 g
 
80-0181_DTT
4482350BS Dithiothreitol (DTT) molecular biology grade 1 g
 
 
4482352BS Dithiothreitol (DTT) molecular biology grade 5 g
 
 
6802335BS Formamide molecular biology grade 500ml
 
 
6812301BS Formamide deionized molecular biology grade 500ml
 
 
0080-0081 Glycine, 500 g
 
0080-0162 Glycine, 2500 g
 
80-0162_Glycine
7132359BS Glycine molecular biology grade 500 g
 
 
7132391BS Glycine molecular biology grade 1kg
 
 
13032356BS MOPS molecular biology grade 100g
 
 
13032357 MOPS molecular biology grade 250g
 
 
20132305BS TAE 10X molecular biology grade 5L
 
 
20132335BS TAE 10X molecular biology grade 500ml
 
 
20142305BS TBE 10X molecular biology grade 5L
 
 
20142335BS TBE 10X molecular biology grade 500ml
 
 
20512305BS TBE 5X molecular biology grade 5L
 
 
20122335BS TE molecular biology grade 500ml
 
 
20122323BS TE molecular biology grade 1L
 
 
20122305BS TE molecular biology grade 5L
 
 
0080-0163 Tricine, 500 g
 
0080-0164 Tricine, 2000 g
 

0080-0077

Tris Base, 500 g
 
0080-0160 Tris Base, 2500 g
 
0080-0078 Tris HCL, 250 g
 
0080-0161 Tris HCL, 1000 g
 
80-0161_Tris_HCl
0080-0083 Tris buffer, 1.5M pH 8.8, 1000 ml
 
20092359BS Tris molecular biology grade 500g
 
 
20092389BS Tris molecular biology grade 5kg
 
 
20092391BS Tris molecular biology grade 1kg
 
 

0080-0180

Urea, 100 g
 
80-0180_Urea
0080-0070 Urea, 1000 g
 
21182391BS Urea molecular biology grade 1000 g
 
 
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