D-Luciferin

For In Vivo Imaging

Quantity: 60 mg

Luciferin is one type of light emitting-pigment that is produced by organisms, such as, Photinus pyralis, the fire fly, which are capable of bioluminescence.  When luciferin is oxidized in the presence of luciferase, a catalyzing enzyme, and ATP, a bluish-green light is produced.  Firefly luciferin is a particularly good reporter for in vivo biophotonic imaging due to the properties of its emission spectra. Luciferin can be used by researchers in a number of ways. It can be used to rapidly monitor light production in vivo, and it can be easily observed using a number of in vivo imaging systems. Because luciferin can penetrate cell membranes, luciferin is easily monitored in animals after injection.  Luciferin is also a valuable tool used to rapidly screen for siRNAs that are influencing cell lines in xenograph mouse models.

Three million H1155luc cells were injected subcutaneous into the mouse flanks. Three weeks following the injection, the mouse was injected with 80ul of 30mg/ml of D-luciferin solution and imaged using the Night OWL instrument. The animal was imaged using a 1ms exposure (Left) and luminescence was detected in the tumors using a 10s exposure (Right).

 

Reference	Description	Qté	Prix unitaire Euros HT
3420-03	MaxSuppressor™ D-Luciferin	60mg	91.00 EUR