Beta-galactosidase ELISA

β-galactosidase is a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides.  In E. coli the β-galactosidase protein is encoded by the lacZ gene which is part of the lac operon.  The β-galactosidase protein is a large (120 kDa, >1000 amino acids) protein that forms a tetramer. The enzyme's function in E. coli is to cleave the disaccharide lactose to form glucose and galactose so they can be used as carbon/energy sources.  The lacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of gene promoters.  β-galactosidase is not typically expressed in mammalian cells, however; β-galactosidase positive staining is a marker for mammalian cellular senescence.  β-galactosidase is a useful reporter gene both in cell culture and in vivo applications due to its ease of detection. 

This ELISA kit contains all the reagents required to quantitate the level of β-galactosidase protein in cells and tissues.  This kit can also be used to study the influence of drug treatments on β-galactosidase staining in animal organs or cells in culture.

 

Reference	Description	Qté	Prix unitaire Euros HT
3420-01	MaxSuppressor™ β-galactosidase ELISA Kit	1 kit	493.00 EUR